Date of Submission
Spring 2023
Academic Program
Chemistry and Biochemistry
Project Advisor 1
Craig Anderson
Project Advisor 2
Swapan Jain
Abstract/Artist's Statement
In this project, a variety of organometallic compounds with Pt(II), Ru(II), or Ir (III) centers were synthesized in order to investigate their interactions with biomolecules with the goal of developing and investigating anticancer agents. Fluorinated ligands were targeted because they have shown promising results in their anticancer ability. Microwave synthetic techniques were employed to synthesize the complexes and they were purified by column chromatography. Two new fluorinated ruthenium (II) compounds were made, ND5400 and RU3FL and one new fluorinated platinum (II) complex, Pt3FL. The complexes were characterized by NMR and IR spectroscopies. Using the CRISPR-Cas9 system, the compounds were evaluated on their effects on enzyme activity and how they inhibit CRISPR. In addition, fluorescent studies were utilized to study if the compounds bind to DNA through groove binding mechanisms.
Open Access Agreement
On-Campus only
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Recommended Citation
Moulene, Anna Mei, "Synthesis and Characterization of Ruthenium (II)-Arene, Platinum (II), and Iridium (III) Complexes and Analysis of Inhibition of CRISPR-Cas9 Model System" (2023). Senior Projects Spring 2023. 24.
https://digitalcommons.bard.edu/senproj_s2023/24
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