Date of Submission

Spring 2023

Academic Program

Chemistry and Biochemistry

Project Advisor 1

Christopher LaFratta

Project Advisor 2

Swapan Jain

Abstract/Artist's Statement

High amounts of biologically active Vitamin B12 are found mainly in animal-based foods and are well known to be absent in fruits and vegetables, with the exception of mushrooms.1 There are studies showing that some wild edible mushrooms, such as black trumpet and chanterelles and cultivated edible mushrooms, contain high amounts of “true” Vitamin B12.1,2 With the increased demand for phytoid -sourced Vitamin B12, mushrooms have the potential to be an additional consumable source of Vitamin B12 since fortified products or Vitamin B12 supplements contain artificial Vitamin B12, cyanocobalamin. The main goal of this project is to show that conventionally cultivated white button and portobello mushrooms (A.bisporus) pose promising Vitamin B12 content that may possibly contribute to the prevention of Vitamin B12 deficiency. In spite of both white button and portobello mushrooms pertaining to the same species, the aim of this thesis was to establish an analytical method for the investigation, determination and comparison of Vitamin B12 in A.bisporus. ICP-OES became the main method used with the assumption that cobalt chloride, free cobalt and cobalt within cobalamin is detected at identical concentration peaks.

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On-Campus only

Creative Commons License

Creative Commons License
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