Date of Submission

Spring 2013

Academic Program

Biology

Project Advisor 1

Brooke Jude

Abstract/Artist's Statement

Due to its promiscuous nature and wide host range, multi-drug resistant (MDR) IncA/C plasmids are regarded as a public health threat. Aquatic environments provide reservoirs where MDR genes and plasmids like IncA/C can be readily exchanged between bacterial species, including animal and human pathogens such as Aeromonas salmonicida and Vibrio cholerae. To better understand the movement and spread of MDR in natural aquatic environments, we examined the frequency of IncA/C plasmid transfer between A. salmonicida strain AS03 and V. cholerae strains C6706 and BAA-2163 (Haitian epidemic isolate) in the presence and absence of chitin. Preliminary data collected in this study suggests C6706 may act as a better recipient for conjugation with AS03 and the transfer of IncA/C while BAA-2163 may not be an adequate recipient. V. cholerae C6706 may represent an ideal plasmid host for IncA/C, acting as a recipient and/or as an intermediate host, transferring IncA/C to other pathogenic bacteria.

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