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Clustered Regularly Interspaced Palindromic Repeats (CRISPR) is a revolutionary gene-editing tool which utilizes a sgRNA and Cas9 enzyme to precisely target and cut a specific region on a DNA strand. In recent years, the popularity of the CRISPR system has grown exponentially, with thousands of papers on the popular gene-editing system published every year. Alongside the emergence of CRISPR, is the rising awareness of the practicality and importance of RNA. New studies on RNA are being conducted at a rate higher than ever, and among them is the study of RNA binding with chemotherapy drugs. In this study, Cisplatin, Oxaliplatin and Carboplatin are used to investigate the effect it has on the CRISPR-Cas9 system when bound to RNA. By incubating sgRNA with these platinum drugs and column purifying it, a sgRNA-drug complex can be made. Using this complex, a Cas9 experiment is run and results analyzed using gel electrophoresis. Analysis shows that by binding Cisplatin or Oxaliplatin to sgRNA, the amount of DNA cleavage is inversely proportional to the concentration of Cisplatin, while the presence of Carboplatin yields no changes.
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Li, Jingzai, "Analysis of Platinum Based Drugs Binding with sgRNA in CRISPR-SpyCas9 Assay" (2022). Senior Projects Spring 2022. 8.
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