Date of Submission

Spring 2019

Academic Programs and Concentrations

Chemistry

Project Advisor 1

Dr. Swapan Jain

Project Advisor 2

Dr. Atahualpa Pinto

Abstract/Artist's Statement

Riboswitches are RNA molecules which respond to specific stimuli by turning on or off transcription or translation. The aptamer domain of the xpt-pbuX riboswitch in B. subtilis has a three-dimensional structure with a specific binding site for guanine. When guanine is bound, the expression platform forms a three-dimensional structure which inhibits transcription of downstream purine synthesis genes. In this work, polyethylene glycol (PEG) was conjugated to position nine of guanine. This will potentially increase the solubility of guanine in solvents such as dimethyl sulfoxide, making it easier to use for assays. The PEGylated guanine will be used to probe the xpt-pbuX riboswitch in an in vivo assay.

Open Access Agreement

Open Access

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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Chemistry Commons

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