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Riboswitches are RNA molecules which respond to specific stimuli by turning on or off transcription or translation. The aptamer domain of the xpt-pbuX riboswitch in B. subtilis has a three-dimensional structure with a specific binding site for guanine. When guanine is bound, the expression platform forms a three-dimensional structure which inhibits transcription of downstream purine synthesis genes. In this work, polyethylene glycol (PEG) was conjugated to position nine of guanine. This will potentially increase the solubility of guanine in solvents such as dimethyl sulfoxide, making it easier to use for assays. The PEGylated guanine will be used to probe the xpt-pbuX riboswitch in an in vivo assay.
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Santana, Leah, "Synthesis of PEG-modified Guanine for Modulation of Transcription via xpt-pbuX Riboswitch" (2018). Senior Projects Spring 2019. 149.