Date of Submission
Academic Programs and Concentrations
Project Advisor 1
Telomere shortening is primarily involved in cell death and cellular aging. However, telomerase can reverse the shortening process and allow cells to overcome apoptosis. Telomerase activity is expressed in 85-90% of cancer cells. Binding of this enzyme can be prevented by the formation of G-quadruplex structures, which include stacks of G-tetrads formed by telomeric TTAGGG tandem repeats. Therefore, G-quadruplex stability is crucial to the inhibition of telomerase activity and ligand-induced quadruplex stabilization emerges as a potential chemotherapeutic pathway due to its high selectivity and specificity towards cancer cells.
Our project aims to understand the stabilization mechanism of DNA/RNA G-quadruplex by organometallic compounds using UV/Vis spectroscopy and Isothermal Titration Calorimetry (ITC). We have shown that quadruplex stability is increased by potassium ions and further enhanced by the cationic porphyrin ligands TMPyP4 and Mn(III) TMPyP4. We also found that the anionic copper (II) phthalocyanine Cu-APC complex, discovered by the Sugimoto group, potentially interacts with both DNA and RNA quadruplexes. The nonionic benzothiophene platinum(II) complex L3, synthesized by the Anderson lab, also interacts with DNA G- quadruplex and potentially stabilizes the structures. Our studies have provided insights into improving quadruplex stability by organometallic ligands and how their stabilizing effects differ due to structural differences.
Open Access Agreement
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Hoang, Hang Thanh, "Stabilization of G-quadruplex Structures by Organometallic Complexes: A Potential Anticancer Mechanism" (2018). Senior Projects Spring 2018. 54.
This work is protected by a Creative Commons license. Any use not permitted under that license is prohibited.Bard Off-campus Download
Bard College faculty, staff, and students can login from off-campus by clicking on the Off-campus Download button and entering their Bard username and password.