Date of Submission

Spring 2012

Academic Program

Biology

Project Advisor 1

Brooke Jude

Abstract/Artist's Statement

An increase in emerging infectious diseases in the last century has sparked an increased need for new antimicrobial compounds to combat illness. While many of these compounds are synthesized, there is also a wealth of possibilities for antimicrobials found in nature, such as the tryptophan derived violacein. Violacein is a purple secondary metabolite produced by Janthinobacterium lividum and thought to act as an antifungal against the deadly cutaneous fungus Batrachochytrium dendrobatidis, which is decimating amphibian populations worldwide. Our study helps to elucidate the biosynthetic pathway of violacein in a Janthinobacterium sp. environmental isolate, and begins to determine if the chemical structure of violacein is unique to its antifungal capabilities. We found that the vioC gene in the violacein operon was interrupted in a green mutant, created through transposon mutagenesis, causing production of chromoviridans, the oxidized form of a stable intermediate in the violacein biosynthetic pathway (deoxychromoviridans). We hoped to test chromoviridans for antifungal abilities against Batrachochytrium dendrobatidis, but contamination issues prevented accurate results from this part of the study.

Distribution Options

Access restricted to On-Campus only

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

This work is protected by a Creative Commons license. Any use not permitted under that license is prohibited.

Bard Off-campus Download

Bard College faculty, staff, and students can login from off-campus by clicking on the Off-campus Download button and entering their Bard username and password.

Share

COinS