Author

Maria Presti

Date of Award

2016

First Advisor

David Myers

Second Advisor

Erin McMullin

Third Advisor

Mira Krendel

Abstract

This thesis seeks to address a single research question in two unique approaches to demonstrate the diversity and non-linearity of research. The role of myo1e on focal adhesion dynamics in podocytes (the role a specific protein has in the movement of specialized epithelial cells in the renal glomerulus) was examined first by determining the effects five different mutations have on the ability of the protein to localize, and second by developing a staining technique for ZONAB, another protein believed to work with myo1e to induce this cellular movement. The rest of the thesis is a general commentary on research, in efforts to shed light about its inner workings to those not familiar with the biomedical field and scientific processes, and an explanation of kidneys and its components.

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